SeMet/ CS纳米复合微球的制备、缓释性能及抑制肿瘤活性
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O627.6

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国家自然科学基金(21461009,81402225,21565013)


Preparation and sustained-release property of SeMet/CS microsphere and its inhibitory effects on cancer cells
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    摘要:

    研究SeMet硒化物的可控缓释系统。以CS为壁材、SeMet为药物模型,采用乳化交联法制备SeMet/ CS纳米复合微球,以单因素实验和正交试验优化制备工艺,结合原子荧光光谱、IR、热重分析及SEM对产物的形貌、结构及性能进行评价和表征,并对其体外缓释和抑制人乳腺癌细胞MCF-7细胞生长等性能进行研究。结果表明:最优工艺条件(0.1%的投药量、2%的交联剂用量、50℃交联10min)制备的SeMet/ CS微球,形貌优良,包封率和载药量分别为31.94%和0.54%,体外缓释硒的性能良好,对MCF-7细胞的生长抑制率随微球缓释时间增加而增加。SeMet/ CS缓释硒复合微球有效避免了硒的突释效应,实现了硒剂量的把控,可作为硒化药物或硒补充剂应用于医疗、食品和精细化工等相关行业。

    Abstract:

    SeMet/CS Nanocomposite microspheres were prepared using SeMet as a core material and chitosan(CS) as a wall material using emulsion crosslinking. The work in this paper focused on the indicators of microspheric form, drug-loading rate, entrapment efficiency and slow-release property using the single-factor and orthogonal test to optimize the preparation technology. During the processes, a scanning electron microscope, a Fourier infrared spectrometer, a thermal analyzer and double-channel atomic fluorescence were applied for detection, analysis and characterization. Furthermore, in vitro tests, such as for the selenium-releasing property and anticancer activity, were studied for the prepared selenium microsphere. The data indicated that the SeMet/CS microsphere prepared under the optimum conditions of 0.1% dosage and 2% dosage of crosslinking agent were crosslinked at 50℃ for 10 min. The obtained SeMet/CS microspheres exhibited superior features, with an Excellent morphology and an embedding ratio and drug loading of 31.94% and 0.59%, respectively. They displayed excellent sustained-release capability in an SBF model. Regarding anticancer activity, they showed a significant inhibitory effect on MCF-7 human breast cancer cells, and there was a positively correlated between the inhibition rate and the content of selenium in the sustained-release liquid. The SeMet/CS microsphere can effectively avoid a burst effect for selenium, allowing a controlled selenium dose. Thus, it can be used in selenide or selenium supplements for further application in medical, food and fine chemical industries.

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引用本文格式: 冉青,商龙臣,吴少尉,李光大,刘信平. SeMet/ CS纳米复合微球的制备、缓释性能及抑制肿瘤活性[J]. 四川大学学报: 自然科学版, 2018, 55: 1057.

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  • 收稿日期:2018-03-20
  • 最后修改日期:2018-04-11
  • 录用日期:2018-04-13
  • 在线发布日期: 2018-09-17
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