Hangzhou Academy of Agricultural Sciences
为了探究双孢蘑菇降温结实的分子机制，本实验以双孢蘑菇W192为对象，在催蕾期利用高通量RNA测序技术对通过4 d、6 d、8 d 把环境温度由21.5 ℃匀速降到17.5 ℃处理后的菌丝体进行基因表达分析.分析结果6 d有1481个差异表达基因，较8 d和4 d分别高出6.9%和34%.GO(Go Ontology)功能聚类分析表明，差异基因在细胞组分和生物学过程中分布较多，其中6 d除了在代谢、细胞过程中差异基因占优外，在胁迫响应、子实体分化和发育过程等条目下的差异基因明显并基本为上调表达，而4 d和8 d并没有出现这类差异基因，同时，6 d的差异表达基因参与的细胞内进行各种氨基酸和糖代谢更明显.KEGG通路（pathway）功能富集分析表明差异表达基因主要富集在氨基酸和抗生素生物合成通路上，其中6 d在糖酵解和核糖体生物合成通路富集，而4 d和8 d未出现.4 d、6 d降温刺激容易高产，但4 d出菇早，密度高，分层不明显等导致菇型受到影响，6 d更加稳产保质.本研究揭示了在不同降温刺激下的菌丝体的表达模式， 可用于指导工厂化双孢蘑菇催蕾期环境调控.
In order to explore the molecular mechanism of Agaricus bisporus cooling and fruiting, this experiment took Agaricus bisporus W192 as the object. High-throughput RNA sequencing technology was used to analyze the gene expression of mycelium treated with the ambient temperature lowered from 21.5 ℃ to 17.5 ℃ at a constant rate for 4 d, 6 d and 8 d. There were 1481 differentially expressed genes in 6 d, which were 6.9% and 34% higher than 8 d and 4 d, respectively. Functional clustering analysis of GO Ontology (GO Ontology) showed that differential genes were more distributed in cellular components and biological processes, of which 6 d was dominant in metabolic and cellular processes. In addition, the differential genes under stress response and fruit body differentiation items were obviously and basically up-regulated. However, no such differentially expressed genes appeared on 4 d and 8 d, and the differentially expressed genes on 6 d were more obviously involved in the metabolism of various amino acids and sugars in cells. Functional enrichment analysis of KEGG pathway showed that the differentially expressed genes were mainly enriched in amino acid and antibiotic biosynthesis pathways, of which 6 d was enriched in glycolysis and ribosomal biosynthesis pathways, while 4 d and 8 d did not. On 4 d and 6 d, the cooling stimulation was easy to produce high yield, but on 4 d, the fruiting was early, the density was high, and the stratification was not obvious which would affect the mushroom type. On 6 d, the yield and quality were more stable. This study revealed the expression patterns of mycelium under different cooling stimuli, which provided a scientific theory for environmental regulation of industrial Agaricus bisporus during bud stimulating stage.
引用本文格式： 陆娜,宋吉玲,闫静,王伟科,周祖法,黄小苏,袁卫东. 工厂化双孢蘑菇不同降温刺激后的转录组分析[J]. 四川大学学报: 自然科学版, 2022, 59: 026002.复制