Pyrrosiapetiolosa was used to make ointment to study its antibacterial and wound healing effects at the animal level. The P.petiolosa was prepared into an ointment, and the rat skin excision trauma model was constructed and randomly divided into normal trauma group and Staphylococcus aureus（S.aureus） infection group. Each group was treated with natural recovery, P. petiolosa ointment and Jingwanhong ointment, and then the weight and eating status of rats, wound healing, wound tissue pathology and serum inflammatory factor expression were measured. The results showed that the body weight and food intake of rats in different treatment groups did not change significantly. Wound healing and histopathological analysis showed that P.petiolosa ointment could promote wound healing in normal wound group and S.aureus infection group. ELISA results showed that P.petiolosa ointment group can significantly reduce the inflammatory response in the early stage of trauma. The results demonstrated that this selfdeveloped Chinese medicinal ointment with P.petiolosa as the main raw material has a good effect of antagonizing S.aureus and promoting wound healing.