高表达lncRNA调控意大利蜜蜂工蜂中肠发育的作用解析
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作者单位:

1.福建农林大学动物科学学院(蜂学学院);2.福建农林大学蜂疗研究所

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S891

基金项目:

国家现代农业产业技术体系建设专项资金(CARS-44-KXJ7); 福建农林大学硕士生导师团队项目(郭睿); 福建省病原真菌与真菌毒素重点实验室开放课题(郭睿);江西省蜜蜂生物学与饲养重点实验室开放基金(JXKLHBB-2020-04)


Analysis of highly-expressed lncRNAs function in regulating midgut development of Apis mellifera ligustica worker
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Affiliation:

1.College of Bee Science, Fujian Agriculture and Forestry University;2.Apitherapy Research Institute, Fujian Agriculture and Forestry University

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    摘要:

    为了探究高表达lncRNA(highly-expressed lncRNA, HlncRNA)调控意大利蜜蜂(Apis mellifera ligustica,简称意蜂)工蜂中肠发育的潜在作用,本研究利用前期获得的组学数据筛选出意蜂7和10日龄工蜂中肠的HlncRNA,通过RT-PCR验证上述HlncRNA的真实表达,通过RT-qPCR分析HlncRNA在1~21日龄工蜂中肠发育过程的表达谱,进而对HlncRNA的调控作用进行生物信息学分析.结果表明7和10日龄工蜂中肠中表达量最高的9条lncRNA相同;这些HlncRNA在1~21日龄工蜂中肠发育过程中真实表达且表达规律不同;HlncRNA通过顺式和反式作用分别调控32个上下游基因和18条共表达mRNA,此外可靶向55个miRNA进而结合131条mRNA.研究结果揭示上述9条HlncRNA可通过顺式作用、反式作用和ceRNA网络潜在调节应激反应与糖类消化和吸收等功能条目和通路,进而调控意蜂工蜂的中肠的发育.

    Abstract:

    In order to explore the potential role of highly-expressed lncRNAs (HlncRNAs) in regulating the midgut development of Apis mellifera ligustica worker, the omics data obtained in the previous at 7- and 10-day-old A. m. ligustica workers’ midguts were used to screen out the HlncRNAs. RT-PCR was performed to confirm the real expression of aforementioned HlncRNAs and analysis the expression pattern of them during the developmental process of 1~21 days old workers’ midguts, and bioinformatic analyses of the regulatory function of HlncRNAs were further performed. The results suggested that the 9 HlncRNAs in the 7- and 10-day-old worker’ s midgut were the same. These HlncRNAs were expressed in 1- to 21-day-old workers’ midguts but their expression patterns were different. HlncRNAs regulated 32 upstream and downstream genes and 18 co-expressed mRNAs via cis-acting and trans-acting manner, respectively. Additionally, these HlncRNAs can target 55 miRNAs and further bind to 131 mRNAs. These results reveal the 9 HlncRNAs potentially regulated functional terms and pathways such as the stress response, sugar digestion and absorption through cis-acting, trans-acting and ceRNA network to regulate the development of A. m. ligustica worker’ s midgut.

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引用本文格式: 王杰,孙明会,隆琦,范元婵,吴鹰,郭意龙,张凯遥,石彩云,陈大福,郭睿. 高表达lncRNA调控意大利蜜蜂工蜂中肠发育的作用解析[J]. 四川大学学报: 自然科学版, 2022, 59: 016005.

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  • 收稿日期:2021-05-05
  • 最后修改日期:2021-05-25
  • 录用日期:2021-06-01
  • 在线发布日期: 2022-01-19
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