盐胁迫下拟南芥去乙酰化酶调控下游基因表达的转录组分析
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复旦大学生命科学学院遗传工程国家重点实验室

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Q946.2

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国家自然科学基金


Transcriptome analysis of downstream gene expression regulated by deacetylase in Arabidopsis thaliana under salt stress
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State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai

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    摘要:

    胁迫是指植物持续地暴露在环境的刺激下,有能力建立保护和适应的机制. 组蛋白的去乙酰化作为一种表观遗传现象,在植物适应环境中发挥了重要的作用. 为了探究高盐胁迫下,组蛋白的去乙酰化酶在植物抵御和适应高盐胁迫中的作用,本研究以拟南芥野生型WT和四突(h2tq,HD2四个基因的突变体)为材料,采用RNA-seq技术和生物信息学方法,对生长10d的幼苗在高盐(150 mmol/L NaCl)处理前和后进行比较转录组分析,并结合实时荧光定量PCR验证转录组数据的可靠性. 结果显示:在高盐处理前WT和hd2q共有25个差异基因,其中上调基因2个,下调基因23个。在高盐处理后,WT和hd2q共有1407个差异基因,其中上调基因772个,下调基因635个. GO和KEGG分析显示,对照的差异基因主要富集在胞外区域和响应脱落酸上,盐处理的差异基因主要富集在胞外区域和细胞壁上. 植物响应盐胁迫是一个涉及不同交叉途径的复杂过程,这些结果可为研究表观遗传在盐胁迫逆境下的刺激响应提供一定参考意义.

    Abstract:

    Stress refers to the ability of plants to establish protective and adaptive mechanisms under continuous exposure to environmental stimuli. As an epigenetic phenomenon, histone deacetylation plays an important role in plant adaptation. In order to explore the role of histone deacetylase in plant resistance and adaptation to high salt stress, wild-type and h2tq (HD2 four mutants) of Arabidopsis thaliana were used in this study. RNA-seq and bioinformatics methods were used to analyze the transcriptome of 10 day old seedlings before and after high salt (150 mmol/L NaCl) treatment, and the reliability of transcriptome data was verified by real-time quantitative PCR. The results showed that there were 25 differentially expressed genes in WT and hd2q, including 2 up-regulated genes and 23 down-regulated genes. After high salt treatment, there were 1407 differentially expressed genes in WT and hd2q, including 772 up-regulated genes and 635 down- regulated genes. Go and KEGG analysis showed that the control genes were mainly enriched in the extracellular region and in response to abscisic acid, while the salt treated genes were mainly enriched in the extracellular region and cell wall. Response to salt stress is a complex process involving different cross pathways. These results can provide some reference for the study of epigenetic response to salt stress.

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引用本文格式: 欧艺鹏,黄轩雅,陈功春,林娟. 盐胁迫下拟南芥去乙酰化酶调控下游基因表达的转录组分析[J]. 四川大学学报: 自然科学版, 2022, 59: 016003.

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  • 收稿日期:2021-04-22
  • 最后修改日期:2021-05-24
  • 录用日期:2021-06-01
  • 在线发布日期: 2022-01-19
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