柠檬醛诱导的拟南芥抗旱性及差异表达基因分析
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四川大学生命科学学院生物资源与环境教育部重点实验室

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Q37

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国家自然科学基金(31071334)


Drought resistance induced by citral in Arabidopsis thaliana and analysis of differentially expressed genes
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Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University

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    摘要:

    本研究旨在探讨柠檬醛在植物抗旱方面的潜在应用价值.以不同浓度的柠檬醛处理拟南芥,我们发现200 μmol/L柠檬醛处理的拟南芥在随后干旱胁迫下的存活率高达80%,而未经柠檬醛处理的对照组只有40%左右.转录组测序(RNA-seq)分析发现经200 μmol/L柠檬醛处理后有230个差异表达基因(differentially expressed genes, DEGs),DEGs在GO功能注释和KEGG通路中分别显著富集为应激反应和内质网中的蛋白质加工,且上调表达的DEGs许多都与热激蛋白家族相关.从中挑选4个(AT1G07400,AT2G26150,AT4G25200,AT4G12400)进行RT-qPCR验证,发现其反映的表达水平和RNA-seq数据一致.本研究表明200 μmol/L柠檬醛处理可以诱导拟南芥产生抗旱性,并可能通过热激蛋白使干旱胁迫后部分解折叠的功能蛋白恢复生理活性状态,从而使拟南芥显示出对干旱更强的耐受性和抵抗力.

    Abstract:

    This study aims to explore the potential application value of citral in the drought resistance of plants. Different concentrations of citral were used to treat Arabidopsis thaliana seedlings, and it was found that the survival rate of Arabidopsis thaliana treated with 200 μmol/L citral under subsequent drought stress was up to 80%, while that of the control group without citral treatment was only about 40%. The results of transcriptome sequencing (RNA-seq) showed that there were 230 differentially expressed genes (DEGs) between seedlings treated with and without 200 μmol/L citral. DEGs were significantly enriched in the response to stimulus and protein processing in endoplasmic reticulum, respectively, in GO term and KEGG pathway, and most of 129 up-regulated genes were related to the heat shock proteins. Four of them (AT1G07400, AT2G26150, AT4G25200, AT4G12400) were selected for RT-qPCR verification, and the gene expression level reflected by RT-qPCR was consistent with the RNA-seq data. The results of this study indicated that the drought resistance was obtained in Arabidopsis thaliana seedlings treated with 200 μmol/L citral, which may be realized by heat shock proteins to restore the physiologically active state of functional proteins that partially unfolded under subsequent drought stress conditions, thereby acquiring better tolerance and resistance to drought stress.

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引用本文格式: 张明迪,白九元,王鑫,罗梅,赵云,张年辉. 柠檬醛诱导的拟南芥抗旱性及差异表达基因分析[J]. 四川大学学报: 自然科学版, 2022, 59: 016001.

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  • 收稿日期:2021-03-18
  • 最后修改日期:2021-04-28
  • 录用日期:2021-04-28
  • 在线发布日期: 2022-01-19
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