利用TRV过表达和沉默系统验证植物油脂合成相关基因的功能
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1.四川大学生命科学学院 生物资源与生态环境教育部重点实验室;2.四川大学生命科学学院生物资源与生态环境教育部重点实验室

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Q945

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农业部转基因重大专项(2016ZX08010001-010)


Plant lipid synthesis-related genes function verification by TRV overexpression and silencing systems
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Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University

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    摘要:

    为探究烟草脆裂病毒过表达和沉默系统进行油脂相关基因研究的可能性,该研究克隆了拟南芥的WRI1和FAD2基因,分别构建了TRV过表达和沉默载体. 利用瞬时侵染技术侵染本氏烟草,取材料进行RT-PCR和脂肪酸含量检测. 结果显示在过表达WRI1基因的本氏烟草中,侵染叶和非侵染叶的WRI1基因及其相关参与脂肪酸合成基因ACP1、KAS1和BCCP2等都有明显上调,且脂肪酸含量检测结果显示分别增加16%和28%. 在沉默FDA2基因的本氏烟草植株中,发现多不饱和脂肪酸含量分别减少约25%和24%. 因此,利用TRV系统对油脂相关基因进行研究是可能的.

    Abstract:

    In order to explore the possibility of tobacco rattle virus overexpression and silencing system for oil-related gene research, this study cloned Arabidopsis thaliana WRI1 and FAD2 genes, and constructed TRV overexpression and silencing vectors, respectively. The transient infection technique was used to infect Nicotiana benthamiana, and the material was collected for RT-PCR and fatty acid content detection. The results showed that the WRI1 gene and related fatty acid synthesis genes ACP1, KAS1, and BCCP2 in both infected and non-infected leaves were significantly up-regulated in Nicotiana benthamiana overexpressing the WRI1 gene, and the fatty acid content test results showed that the levels of fatty acids increased 16% and 28%, respectively. In Nicotiana benthamiana plants that silenced the FDA2 gene, the content of polyunsaturated fatty acids was found to be reduced by approximately 25% and 24%, respectively. Therefore, it is possible to use TRV system to study lipid related genes.

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引用本文格式: 江悦,谢桂兰,陈放,徐莺. 利用TRV过表达和沉默系统验证植物油脂合成相关基因的功能[J]. 四川大学学报: 自然科学版, 2021, 58: 066003.

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  • 收稿日期:2021-03-12
  • 最后修改日期:2021-04-01
  • 录用日期:2021-04-08
  • 在线发布日期: 2021-12-12
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