National Engineering Research Center of Juncao Technology, Fujian Agriculture and Forestry University
为探明以鲜菌草为主要原料工厂化栽培银耳的机理，利用气相色谱-质谱联用法分析银耳菌丝生长期G1（接种后12 d）、原基分化期G2（接种后24 d ）和子实体生长期G3（接种后36 d ）的物质转化特性，经主成分、偏最小二乘法判别、正交偏最小二乘法判别分析，结果显示， G2比G1 、G3比G2 、G3比G1分别有426、423和466种差异代谢物；G2与G1的糖类含量高于G3；G3比G 1的脂肪类、萜类等含量增加；对差异代谢物进行筛选和代谢通路匹配，差异途径为脂肪酸合成、乙醛酸和二羧酸代谢及柠檬酸循环，G2与G1、G3与G2、 G3与G1分别在不饱和脂肪酸，丙氨酸、天冬氨酸、谷氨酸，甘油酯代谢存在明显差异. 以上实验结果表明，鲜菌草工厂化栽培银耳不同生长期物质转化及代谢物质差异明显.
In order to explore the mechanism of factory cultivation of Tremella fuciformis with fresh juncao as the main substrate, GC-MS was used to analyze the substance transformation characteristics during 3 different stages，which were G1 (hypha growth phase, 12 d after inoculation), G2 (primordium differentiation stage, 24 d after inoculation) and G3 (fruit body growth phase, 36 d after inoculated). After main component analysis, partial least squares discriminant analysis, and orthogonal partial least squares discriminant analysis, the results showed that there were 426, 423, and 466 different metabolites in G2 than G1, G3 than G2, and G3 compared to G1, respectively. The carbohydrate content in G2 and G1 was higher than that in G3. The content of fat and terpenoids increased in G3 than that in G1. Differential metabolites were screened and metabolic pathways were matched. The differential pathways were fatty acid synthesis, glyoxylic acid and dicarboxylic acid metabolism, and the citric acid cycle. The synthesis pathways of unsaturated fatty acids were significantly different between G2 and G1. The metabolic pathways of alanine, aspartic acid and glutamate were significantly different between G3 and G2.The metabolic pathways of glycerol metabolism were significantly different between G3 and G1. All the results indicated that there were significant differences in substance transformation and metabolites in different growth stages of Tremella fuciformis.
引用本文格式： 林兴生,王泽辉,马运龙,林占熺,林辉,林冬梅,罗海凌. 鲜菌草工厂化栽培银耳物质转化特性研究[J]. 四川大学学报: 自然科学版, 2021, 58: 056001.复制