灵芝抑制肿瘤微环境中Treg细胞功能的机制
作者:
作者单位:

四川省中医药科学院菌类药材研究所, 菌类药材系统研究与开发实验室, 中药材品质及创新中药研究四川省重点实验室

作者简介:

通讯作者:

中图分类号:

R96

基金项目:

四川省公益性科研院所基本科研业务专项(A2018N18); 四川省十三五中药材育种攻关项目(2016NYZ0036); 国家现代农业产业技术体系四川道地中药材创新团队项目(SCCXTD-2020-19)


Effect of Ganoderma lucidum on inhibition of Treg cell function in mouse tumor microenvironment
Author:
Affiliation:

Sichuan Academy of Chinese Medicine Sciences, Research and Development Laboratory for Fungal Medicinal Systems, Research on the Quality of Chinese Medicinal Materials and Innovative Traditional Chinese Medicine Sichuan Key Laboratory

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为探究灵芝抑制肿瘤的可能机制,分别采用灵芝三萜、多糖及三萜多糖配伍对H22荷瘤小鼠模型进行干预,检测瘤体重量、体积,流式细胞法检测肿瘤组织中Treg细胞比例,RT-PCR法检测趋化因子CCL22、受体CCR4和FOXP3 mRNA表达水平,IHC法检测FOXP3蛋白表达水平,ELISA法检测IL-10、TGF-β1、IL-2含量.结果显示,灵芝能抑制H22肿瘤生长,配伍组抑瘤率(47.8%)明显高于三萜组(21.4%)和多糖组(30.8%);灵芝能降低肿瘤微环境中Treg细胞比例,配伍组比例(1.93%)明显低于三萜组(3.04%)和多糖组(2.18%);灵芝能显著降低相关mRNA的表达、FOXP3和IL-10、TGF-β1水平,增加IL-2水平.因此,灵芝可通过减少Treg细胞向肿瘤组织浸润、抑制其分泌细胞因子,同时增加IL-2水平来促进抗肿瘤免疫,且三萜和多糖合用可能存在协同作用.

    Abstract:

    To investigate the possible mechanism of Ganoderma lucidum inhibiting tumor,the H22 tumorbearing mice were treated with triterpenoids, polysaccharides and their mixture separately. The tumor weight and volume were measured, and the proportion of Treg cells in tumor tissues was detected by flow cytometry. The expression level of CCL22、CCR4 and FOXP3 were detected by RT-PCR. Immunohistochemical staining was used to detect the expression level of FOXP3 protein, ELISA was used to detect the cytokine content of IL-10, TGF-β1 and IL-2. The results showed the tumor inhibition rate in the mixture group(47.8%) was significantly higher than that in the triterpenoid group (21.4%) and the polysaccharide group (30.8%) . The proportion of Treg cells of the mixture group (1.93%) in the tumor microenvironment was significantly lower than that in the triterpenoid group (3.04%) and polysaccharide group (2.18%). The levels of FOXP3, IL-10, TGF-β1 and related mRNA were significantly reduced in Ganoderma lucidum groups. Meanwhile, the IL2 level was increased in Ganoderma lucidum groups. Therefore, Ganoderma lucidum can inhibit Treg cell function by reducing the infiltration of Treg cells into tumor tissue and inhibiting cytokine secretion in the mouse tumor microenvironment. Meanwhile, Ganoderma lucidum can increase the level of IL-2 in the tumor microenvironment to promote an antitumor immune response. Besides, the combination of triterpenoids and polysaccharides may have a synergistic effect.

    参考文献
    相似文献
    引证文献
引用本文

引用本文格式: 李芳,余梦瑶,江南,贺黎铭,姚珂,罗霞. 灵芝抑制肿瘤微环境中Treg细胞功能的机制[J]. 四川大学学报: 自然科学版, 2021, 58: 026003.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2020-08-14
  • 最后修改日期:2020-10-20
  • 录用日期:2020-10-31
  • 在线发布日期: 2021-04-02
  • 出版日期: