本实验以1/5的Hoagland溶液为培养液，设置0、20、40、60、80、100 mL/L六个铜绿微囊藻（FACHB 930，107 cells/mL）接种梯度与少根紫萍（ZH0051，400 g/m2）共培养.结果发现，少根紫萍的生长速率、抗氧化物酶活性（SOD、APX和CAT）以及培养液中微囊藻毒素含量随着铜绿微囊藻接种量的增加呈现出先升高后降低的趋势，而少根紫萍体内微囊藻毒素却先降低后升高，淀粉累积量呈现出逐渐下降的趋势.铜绿微囊藻的接种量为20 mL/L时，少根紫萍的生长速率达到最大值（3.64 g/m2·d）.当接种量在60~80 mL/L之间时，少根紫萍能从培养液中高效的移除氮、磷.实验结果表明，当水体中铜绿微囊藻浓度在2.0×108 cells/L（接种量为20 mL/L）左右时，少根紫萍能够达到最大的生物量、相对高的淀粉累积量、以及高品质的氮磷去除率，并能有效地抑制铜绿微囊藻的生长.
In this experiment, 0, 20, 40, 60, 80 and 100 mL/L of Microcystis aeruginosa(FACHB 930, 107 cells/mL) were cocultured with Spirodela punctata(ZH0051, 400 g/m2) in Hoagland which was diluted five times. Experimental results showed that the growth rate and the antioxidant enzyme activities (SOD, APX and CAT) of Spirodela punctata and the microcystins in the culture medium increased first and decreased with the increase of inoculation volume of Microcystis aeruginosa. In contrast, the content of microcystins inSpirodela punctata decreased first and then increased, and the accumulation of starch in the Spirodela punctata had showed a gradual decline. When the inoculation of Microcystis aeruginosa was 20 mL/L, the growth rate of Spirodela punctata reached its maximum value (3.64 g/m2·d). When the inoclum size was between 60~80 mL/L, the Spirodela punctata was able to remove nitrogen and phosphorus efficiently from the culture medium. It’s showed that when the concentration of Microcystis aeruginosa in water was about 2.0×108 cells/L (the inoculation level was 20 mL/L),Spirodela punctata can achieve a maximum biomass, relatively high starch accumulation rate, and high quality of nitrogen and phosphorus removal rate, and can effectively inhibit the growth of Microcystis aeruginosa.
引用本文格式： 孙焕,苏稚喆,冯婷,赵云,魏炜. 铜绿微囊藻与浮萍联合生长的生物学效应及其对污水环境指标的影响[J]. 四川大学学报: 自然科学版, 2018, 55: 853.复制