瑞香狼毒对东亚飞蝗作用机理的初步研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

S43

基金项目:

国家自然科学基金(31272068) ;四川省应用基础研究项目(2014JY0063)


Studies on the mechanism of Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen)
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    利用硅胶柱分离瑞香狼毒乙酸乙酯萃取物得到I、II、III段粗分物,并测定对东亚飞蝗毒杀活性最高的I段粗分物对东亚飞蝗的致死性及对其体内Na+-K+-ATPase、Ca2+/Mg2+-ATPase、乙酰胆碱酯酶的抑制活性. 研究表明:瑞香狼毒I段粗分物对东亚飞蝗具有较强的毒杀作用,在药物浓度为10mg/mL、72h对东亚飞蝗的校正死亡率为92.5%;同时,毒杀作用具有浓度和时间依赖性;而且瑞香狼毒乙酸乙酯萃取物I段粗分物对上述3种酶均有一定的抑制作用,且对Na+-K+-ATPase的抑制作用最强,在药物浓度为5mg/mL、96h对Na+-K+-ATPase的抑制率为40.35%;分析发现瑞香狼毒活性成分对东亚飞蝗的毒杀作用与对Na+-K+-ATPase的抑制作用之间具有显著相关性,据此推测,Na+-K+-ATPase可能是瑞香狼毒活性成分的主要作用靶标.

    Abstract:

    Using silica gel column to separate the ethyl acetate extract of Stellera chamaejasme L. we can get I, II, III sections. The activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase in insects were determined in vivo. The results showed that I section from Stellera chamaejasme L. had the strongest poison effect on Locusta migratoria manilensis (Meyen). Under the concentration of 10 mg/mL, the corrected mortality was 92.5% in 72 hour. And the poison effect there was persistence. The I section from Stellera chamaejasme L. also had certain inhibition on activity of Na+-K+-ATPase、Ca2+/Mg2+-ATPase、acetylcholinesterase. The inhibiton rate of Na+-K+-ATPase was the highest among the three kinds of enzymes. There was a well correlation between the toxic action of the active ingredients from Stellera chamaejasme L. to Locusta migratoria manilensis (Meyen) and the inhibition on activity of Na+-K+-ATPase in Locusta migratoria manilensis (Meyen). Na+-K+-ATPase is likely the target of the active ingredients from Stellera chamaejasme L. against Locusta migratoria manilensis (Meyen).

    参考文献
    相似文献
    引证文献
引用本文

引用本文格式: 陈琼,高甜甜,李波,查艳梅,陶科,金洪,侯太平. 瑞香狼毒对东亚飞蝗作用机理的初步研究[J]. 四川大学学报: 自然科学版, 2017, 54: 665.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2016-03-16
  • 最后修改日期:2016-04-29
  • 录用日期:2016-05-03
  • 在线发布日期: 2017-06-03
  • 出版日期: