成都动物园野生动物源贾第虫的多位点基因分型鉴定
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国家自然科学基金,其它


Multilocus genotype (MLG) analysis of Giardia from captive wildlife in Chendu zoo
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    摘要:

    为了解四川省成都市动物园野生动物贾第虫的流行及基因型,本研究采集了146份不同野生动物的新鲜粪便并提取基因组DNA。通过巢式PCR扩增β-giardin、tpi和gdh基因,扩增产物测序后进行种系发育分析。结果表明,CDZOO1黇鹿源和CDZOO3龟源贾第虫通过多位点基因分型(MLG)鉴定为AI-1亚型;CDZOO2鹿源贾第虫为E型(β-giardin基因位点);CDZOO4黇鹿源贾第虫为A型(β-giardin和tpi基因位点);CDZOO5浣熊源和CDZOO6细尾獴源贾第虫在β-giardin位点为D型而在tpi位点为A型。本研究首次对成都动物园野生动物做了贾第虫分子流行病学调查,并采用MLG法鉴定龟和黇鹿源贾第虫基因型,同时发现人畜共患贾第虫A型感染野生动物,具有重要的公共卫生学意义。

    Abstract:

    In order to investigate the infection and genotypes of Giardia from different wildlife in Cheng Du zoo, a total of 146 fresh fecal samples were collected and their genome DNA extracted. The β-giardin, tpi and gdh genes were amplified by nested-PCR and the product were sequenced followed by phylogenetic analysis. As a result, two persian fallows (CDZOO1 and CDZOO4), a deer (CDZOO2), a tortoise (CDZOO3), a raccoon (CDZOO5), a meerkat (CDZOO6) were infected with Giardia. Multilocus genotypes (MLGs) identified assemblages AI-1 in CDZOO1 and CDZOO3; CDZ002 was infected with assemblage A in both β-giardin and tpi loci; CDZOO4 was confirmed as assemblage A at the tpi locus; CDZOO5 and CDZOO6 were identified as assemblage D at the β-giardin locus while assemblage A based on the tpi locus. In conclusion, this is the first report of molecular epidemiology of Giardia in Chengdu zoo. Meanwhile, Giardia from a tortoise and persian fallow are analyzed by an MLG technique targeting three genes (β-giardin, tpi, and gdh). Furthermore, assemblage A maintained in wildlife and transmitted between them have a zoonotic potential.

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引用本文格式: 李威,屈羽,钟志军,杨平,李云娇,王吴优,刘学涵,谢娜,邓家波,彭广能. 成都动物园野生动物源贾第虫的多位点基因分型鉴定[J]. 四川大学学报: 自然科学版, 2017, 54: 185.

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  • 收稿日期:2015-05-26
  • 最后修改日期:2016-03-17
  • 录用日期:2016-03-22
  • 在线发布日期: 2017-01-21
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