目的 制备具有推-拉电子结构的苯并吡喃衍生物BPAD-2，并初步评价探针的荧光性质、Abeta标记染色和血脑屏障通过能力。方法 以邻羟基苯乙酮为初始原料，通过缩合反应合成了BPAD-2，并通过1H-NMR与MS确认结构；考察所制备探针的荧光光谱；通过荧光染色考察探针的Aβ结合标记；通过荧光成像考察探针的血脑屏障通过能力。结果 BPAD-2的最大发射波长为658 nm；BPAD-2荧光标记的Abeta呈亮红色；BPAD-2尾静脉注入正常小鼠后脑内显示荧光信号，10 min内荧光强度达到最高，随着时间延长荧光强度逐渐减弱。结论 苯并吡喃结构的BPAD-2具有近红外荧光性质，并显示良好的Aβ荧光标记和血脑屏障通过能力。
OBJECTIVE We synthesized a benzopyrane derivative BPAD-2 with push-pull electronic structure, and assessed its fluorescence properties, labelling ability for beta-amyloid plaques, and blood-brain barrier penetrating ability. METHODS BPAD-2 was synthesized by condensation reaction with 1-(2-hydroxy-phenyl)-ethanone as the primary reagent, and confirmed by 1H-NMR and MS. Fluorescence spectra were observed; fluorescence staining was performed to evaluate labelling ability for beta-amyloid plaques; fluorescent imaging was performed to evaluate blood-brain barrier penetrating ability. RESULTS The emission maximum of BPAD-2 in PBS was at 658 nm; BPAD-2 showed bright red labelling of beta-amyloid plaques; BPAD-2 penetrated the blood-brain barrier and peaked at 10 min postinjection and showed reasonable washout from the brain. CONCLSION BPAD-2 showed specific labelling of beta-amyloid plaques and had good blood-brain barrier penetrating ability.
引用本文格式： 程妍,欧阳向硕,梁田,张志荣. 用于Aβ标记的新型近红外荧光染料BPAD-2的制备与生物学评价[J]. 四川大学学报: 自然科学版, 2016, 53: 193.复制